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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva <t>RPN800E,</t> 12 to 225 kDa)
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SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva RPN800E, 12 to 225 kDa)

Journal: Applied Microbiology and Biotechnology

Article Title: DNA methylases for site-selective inhibition of type IIS restriction enzyme activity

doi: 10.1007/s00253-024-13015-7

Figure Lengend Snippet: SDS-PAGE showing the expression of M2. Eco 31I (KPL01 and KPL02) at different IPTG concentrations. Lanes: P, pre-induction; 1, 0.005 mM; 2, 0.05 mM; 3, 0.5 mM; and 4, 1 mM. MW, molecular weight marker (Amersham™ ECL™ Rainbow™ Marker - Full range, Full Range, Cytiva RPN800E, 12 to 225 kDa)

Article Snippet: AmershamTM ECLTM RainbowTM Marker - Full range RPN800E, 12 to 225 kDa (Cytiva, Amersham, UK), and PageRulerTM Plus Prestained Protein Ladder, 10 to 250 kDa (Thermo Fisher Scientific), were used as molecular weight markers.

Techniques: SDS Page, Expressing, Molecular Weight, Marker